NASC

NASC ID: N849130

Name: WiscDsLox5D01

ABRC stock number: CS849130

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

Wiscseq_DsLox5D01.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G67000.1 (chr 1 pos 25004740 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG810043.

Wiscseq_DsLox5D01.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G15840.1 (at chr 1 pos 5451121 (C/5451121-5451166) on the TAIR10). The sequences' 1-46 bps mapped on the genome, 86-100 bps onto the T-DNA. Pools P 0, R 1, C 4 N 3. The insertion site is at the 3' end of the sequence mapping. Genbank ID: KO453158.

Donation Date: 2004-12-01

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox5D01

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
	WiscDsLox5D01	WiscDsLox5D01	AT1G15840 At1g67000

EMBL accession:

CW792791